Putting an accurate mass on a lyophilized peptide is harder than the task appears. What makes it awkward is that the errors are systematic instead of random: they lean one way…
Once a peptide is in solution the clock is running. The practical question is how to draw from that solution across the working life of an experiment without degrading whatever…
Most of the water leaves a peptide preparation during freeze-drying. All of it does not. Whatever remains is simultaneously a mass appearing on your invoice and a variable acting…
One word, several unrelated chemistries. "Degradation" bundles together processes that attack different residues, run at different rates and leave different fingerprints…
Experiment records tend to be meticulous. Material records tend not to be. The asymmetry only becomes visible when a result looks wrong…
Take a vial straight out of the refrigerator, break the seal, and room air meets a surface sitting below its dew point. Moisture condenses onto the powder immediately.
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